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Angelman syndrome-associated point mutations in the Zn-binding N-terminal (AZUL) domain of UBE3A ubiquitin ligase inhibit binding to the proteasome.
Kuhnle S, Martinez-Noel G, Leclere F, Hayes SD, Harper JW, Howley PM
J Biol Chem. 2018 Sep 26. pii: RA118.004653. doi: 10.1074/jbc.RA118.004653.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
119304PHAGE-P CMVt N-HA hE6-AP I (C21Y)lentiviral vector for expression of HA-Tagged E6-AP I (C21Y) in mammalian cells
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119308PHAGE-P CMVt N-HA hE6-AP I (G20V)lentiviral vector for expression of HA-Tagged E6-AP I (G20V) in mammalian cells
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119309PHAGE-P CMVt N-HA hE6-AP I (R39H)lentiviral vector for expression of HA-Tagged E6-AP I (R39H) in mammalian cells
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119310PHAGE-P CMVt N-HA hE6-AP I (AA 1-64)lentiviral vector for expression of HA-Tagged E6-AP I AA 1-64 in mammalian cells
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119311PHAGE-P CMVt N-HA hE6-AP I (AA 1-376)lentiviral vector for expression of HA-Tagged E6-AP I AA 1-376 in mammalian cells
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119312PHAGE-P CMVt N-HA hE6-AP I (AA 1-500)lentiviral vector for expression of HA-Tagged E6-AP I AA 1-500 in mammalian cells
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119313PHAGE-P CMVt N-HA hE6-AP I (AA 55-376)lentiviral vector for expression of HA-Tagged E6-AP I AA 55-376 in mammalian cells
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119314PHAGE-P CMVt N-HA hE6-AP I (AA 55-852)lentiviral vector for expression of HA-Tagged E6-AP I AA 55-852 in mammalian cells
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119315PHAGE-P CMVt N-HA hE6-AP I (AA 366-852)lentiviral vector for expression of HA-Tagged E6-AP I AA 366-852 in mammalian cells
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119316PHAGE-P CMVt N-HA hE6-AP I (AA 481-852)lentiviral vector for expression of HA-Tagged E6-AP I AA 481-852 in mammalian cells
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119317PHAGE-P CMVt N-HA hE6-AP II (AA 1-87)lentiviral vector for expression of HA-Tagged E6-AP I AA 1-87 in mammalian cells
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119318PHAGE-P CMVt N-HA hE6AP III (C41A, C46A, C840A)lentiviral vector for expression of HA-Tagged E6-AP III (C41A, C46A, C840A) in mammalian cells
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119319PHAGE-P CMVt N-HA hE6-AP III (AA 1-84)lentiviral vector for expression of HA-Tagged E6-AP III AA 1-84 in mammalian cells
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