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Noncanonical autophagy at ER exit sites regulates procollagen turnover.
Omari S, Makareeva E, Roberts-Pilgrim A, Mirigian L, Jarnik M, Ott C, Lippincott-Schwartz J, Leikin S
Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10099-E10108. doi: 10.1073/pnas.1814552115. Epub 2018 Oct 4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
119826eGFP-proα2(I)Expresses mouse Type I procollagen α2 chain (Col1a2) with GFP between signal sequence and exon 6
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119827eGFP-proα2(I)-G610CExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and GFP between signal sequence and exon 6
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119836mCerulean-proα2(I)Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6
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119837mCerulean-proα2(I)-G610CExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mCerulean between signal sequence and exon 6
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119838mVenus-proα2(I)Expresses mouse Type I procollagen α2 chain (Col1a2) with mVenus between signal sequence and exon 6
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119839mVenus-proα2(I)-G610CExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mVenus between signal sequence and exon 6
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119840mApple-proα2(I)Expresses mouse Type I procollagen α2 chain (Col1a2) with mApple between signal sequence and exon 6
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119841mApple-proα2(I)-G610CExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mApple between signal sequence and exon 6
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119842mCerulean-proα1(I)Expresses mouse Type I procollagen α1 chain (Col1a1) with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
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119843eGFP-proα1(I)Expresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
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119844mApple-proα1(I)Expresses mouse Type I procollagen α1 chain (Col1a1) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
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119845mVenus-proα1(I)Expresses mouse Type I procollagen α1 chain (Col1a1) with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
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120160eGFP-proα2(I)-LExpresses mouse Type I procollagen α2 chain (Col1a2) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
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120161mApple-proα2(I)-LExpresses mouse Type I procollagen α2 chain (Col1a2) with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
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Antibodies from Article