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OVOL1 Influences the Determination and Expansion of iPSC Reprogramming Intermediates.
Kagawa H, Shimamoto R, Kim SI, Oceguera-Yanez F, Yamamoto T, Schroeder T, Woltjen K
Stem Cell Reports. 2018 Dec 28. pii: S2213-6711(18)30527-7. doi: 10.1016/j.stemcr.2018.12.008.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
120352PB-TAC-Cas9piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.
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120353PB-TAC-OKMS-Cas9piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.
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120354PB-TAC-OK+9MS-Cas9piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.
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120355PB-TAC-KRAB-dCas9piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.
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120356PB-TAC-OKMS-KRAB-dCas9piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.
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120357PB-TAC-OK+9MS-KRAB-dCas9piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.
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120358PB-U6piggyBac transposon vector for sgRNA expression.
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120359pENTR-mOvol1Gateway entry vector containing mouse Ovol1.
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120360PB-TAB-mOvol1piggyBac transposon for dox-inducible expression of Ovol1 (with Bgeo; ie. LacZ / NeoR fusion).
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