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A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.
Wright AV, Wang JY, Burstein D, Harrington LB, Paez-Espino D, Kyrpides NC, Iavarone AT, Banfield JF, Doudna JA
Mol Cell. 2019 Feb 21;73(4):727-737.e3. doi: 10.1016/j.molcel.2018.12.015. Epub 2019 Jan 29.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
120364pET-3a-His-MBP-TEV-VC_Cas1_WTExpresses wildtype V-C Cas1
120365pET-3a-His-MBP-TEV-VD_Cas1_WTExpresses wildtype V-D Cas1
120366pVC-CRISPR1Plasmid containing shortened version of upstream V-C CRISPR array for integration assays
120368pVC_CRISPR_CatReporter plasmid for full-site selection assay
120369pVC-CRISPRPlasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library
120370pVD_CRISPRPlasmid containing shortened version of V-D CRISPR array for integration assays

Recombinant Antibodies from Article