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Structural Basis for Cholesterol Transport-like Activity of the Hedgehog Receptor Patched.
Zhang Y, Bulkley DP, Xin Y, Roberts KJ, Asarnow DE, Sharma A, Myers BR, Cho W, Cheng Y, Beachy PA
Cell. 2018 Nov 15;175(5):1352-1364.e14. doi: 10.1016/j.cell.2018.10.026. Epub 2018 Nov 8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
120886pVLAD6-mmPtch1-B-SBPBacMam vector for Ptch1-B expression
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120887pVLAD6-mmPtch1-B-NNQ-TEV-SBPBacMam vector for Ptch1-B NNQ variant expression
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120888pcDNA-h-mmPtch1-BExpresses Ptch1-B for function assays
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120889pcDNA-h-mmPtch1-FLExpresses wildtype Ptch1 for function assays
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120902pcDNA-h-mmPtch1-B-HAExpresses Ptch1-B for function assays
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120903pcDNA-h-mmPtch1-B-HA-GFPExpresses Ptch1-B for function assays
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120904pcDNA-h-mmPtch1-B-Y233C-HAExpresses Ptch1-B (Y233C) for function assays
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120905pcDNA-h-mmPtch1-B-L234C-HAExpresses Ptch1-B (L234C)
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120906pcDNA-h-mmPtch1-B-L235C-HAExpresses mmPtch1-B (L235C)
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120907pcDNA-h-mmPtch1-B-I766FV111F-HA-GFPExpresses Ptch1-B (I766FV111F)
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120908pcDNA-h-mmPtch1-B-V111FL114FW115A-HA-GFPExpresses Ptch1-B (V111FL114FW115A)
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120909pcDNA-h-mmPtch1-B-I766FV111FL114F-HA-GFPExpresses Ptch1-B (I766FV111FL114F)
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120910pcDNA-h-mmPtch1-B-HA-TagBFPExpresses BFP tagged Ptch1-B
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120911pcDNA-h-mmPtch1-B-V111FL114FI766F-HA-TagBFPExpresses BFP tagged Ptch1-B (V111FL114FI766F)
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120921pcDNA-h-mmPtch1-FL-TagBFPExpresses BFP tagged Ptch1
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121135pHTSHP-Smt-IIShhNExpresses N-terminal SUMO tagged II-ShhN in bacteria
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121136pHTSHP-ShhN-CysExpresses ShhN with a C-terminal cysteine residues in bacteria
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121164pcDNA-h-mmPtch1-B-NNQ-HA-GFPExpresses mmPtch1-B NNQ variant
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