Addgene: Generating stable cell lines with quantifiable protein production using CRISPR/Cas9-mediated knock-in.

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Brian Chen
Lo et al

Generating stable cell lines with quantifiable protein production using CRISPR/Cas9-mediated knock-in.
Lo CA, Greben AW, Chen BE
Biotechniques. 2017 Apr 1;62(4):165-174. doi: 10.2144/000114534. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
121448	ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP	To make a stable human recombinant cell line; see right thumbnail map image for PQR annotations
165043	RPL13A_PQR_mScarlet_NOL_LoxP_PQR_zeo_PQR_MCS_RPL13A-3'UTR	Repair template for CAS9 complex genome editing

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