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Engineering of CRISPR-Cas12b for human genome editing.
Strecker J, Jones S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F
Nat Commun. 2019 Jan 22;10(1):212. doi: 10.1038/s41467-018-08224-4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
122445pZ143-pcDNA3-BvCas12bExpresses BvCas12b in mammalian cells
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122446pZ149-pcDNA3-BhCas12b_v4Expresses BhCas12b_v4 in mammalian cells
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122447pZ147-BvCas12b-sgRNA-scaffoldExpresses sgRNA for BvCas12b in mammalian cells
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122448pZ148-BhCas12b-sgRNA-scaffoldExpresses sgRNA for BhCas12b in mammalian cells
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158615TwinStrep-SUMO-NLS-BhCas12b_v4For protein expression of BhCas12b_v4
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