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SCAF4 and SCAF8, mRNA Anti-Terminator Proteins.
Gregersen LH, Mitter R, Ugalde AP, Nojima T, Proudfoot NJ, Agami R, Stewart A, Svejstrup JQ
Cell. 2019 May 10. pii: S0092-8674(19)30457-X. doi: 10.1016/j.cell.2019.04.038. PubMed Article

Plasmids from Article

ID Plasmid Purpose
122464pDONR223 SCAF4pDONR for Gateway cloning
122465pDONR223 SCAF4_CRISPR resistantpDONR for Gateway cloning
122466pDONR223 SCAF8_isoC_CRISPR resistantpDONR for Gateway cloning
122467pDONR223 SCAF8_isoDpDONR for Gateway cloning
122468pDONR223 SCAF8_isoD_CRISPR_resistantpDONR for Gateway cloning
122469pFRT/TO/SCAF4-FLAGDox-inducible FLAG (C-terminal tag) SCAF4 expression
122470pFRT/TO/FLAGHA-SCAF8Dox-inducible FLAG/HA (N-terminal tag) SCAF8 expression
122471pFRT/TO/GFP-SCAF4 (CRISPR_resistant)Dox-inducible GFP (N-terminal tag) SCAF4 expression
122472pFRT/TO/GFP-SCAF8 (CRISPR_resistant)Dox-inducible GFP (N-terminal tag) SCAF8 expression

Antibodies from Article