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A Tet/Q Hybrid System for Robust and Versatile Control of Transgene Expression in C. elegans.
Mao S, Qi Y, Zhu H, Huang X, Zou Y, Chi T
iScience. 2019 Jan 25;11:224-237. doi: 10.1016/j.isci.2018.12.023. Epub 2018 Dec 27. PubMed Article

Plasmids from Article

ID Plasmid Purpose
122613TC358TRE-delta-pes-10-GFP
122614TC374Prpl-28::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1
122615TC512Pmig-13::Zip(-)::QFAD::NLS::SL2::mCherry
122616TC513Punc-4c::SV40 NLS::rtetR::Zip(+)::SL2::mCherry
122618TC562Punc-4::rtetR-QFAD(650-777+790-816)
122619TC690Punc4::QF2::QS::mKate
122621TC812QUAS::CFP
122625TC393Pser-2::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1
122629TC476Punc-54::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1
164885TC358TRE::GFP, inducible GFP expression when co-express with rtTA
164886TC374Prpl28::rtetR-QFAD::p2A::mKate::T2A::tetR-pie1,
164887TC562Punc-4::rtetR-QFAD(650-777+790-816)::P2A::mKate::T2A::tetR-pie1
164888TC512Pmig-13::Zip(-)::QFAD::NLS::SL2::mCherry
164889TC513Punc-4c::SV40NLS::rtetR::Zip(+)::SL2::mCherry
164891TC550Punc-4::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1
164892TC646Pdpy30::loxP::mcherry::let-858terminator::loxP::GFP
164893TC811TRE::YFP, inducible YFP expression when co-express with rtTA
164894TC812QUAS::CFP, express CFP when co-express with QF
164895TC872TRE::nCre, inducible nCre expression when co-express with rtTA

Antibodies from Article