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Deciphering and engineering chromodomain-methyllysine peptide recognition.
Hard R, Li N, He W, Ross B, Mo GCH, Peng Q, Stein RSL, Komives E, Wang Y, Zhang J, Wang W
Sci Adv. 2018 Nov 7;4(11):eaau1447. doi: 10.1126/sciadv.aau1447. eCollection 2018 Nov.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
138250pcDNA3-CBX1mut-DronpaTriple-mutant CBX1 chromodomain (residues 20-73) tagged with reversibly switchable FP Dronpa. Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.
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138251pcDNA3-CBX1mut-PA-mCherryTriple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.
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138252pcDNA3-CBX1wt-PA-mCherryWild-type CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry).
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Antibodies from Article