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A Singular System with Precise Dosing and Spatiotemporal Control of CRISPR-Cas9.
Manna D, Maji B, Gangopadhyay SA, Cox KJ, Zhou Q, Law BK, Mazitschek R, Choudhary A
Angew Chem Int Ed Engl. 2019 May 6;58(19):6285-6289. doi: 10.1002/anie.201900788. Epub 2019 Apr 2.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
124522pX330-NL-DHFR-SpCas9Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells
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124523pX330-NLC-DHFR-SpCas9Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells
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