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Development of hRad51-Cas9 nickase fusions that mediate HDR without double-stranded breaks.
Rees HA, Yeh WH, Liu DR
Nat Commun. 2019 May 17;10(1):2212. doi: 10.1038/s41467-019-09983-4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
125561hRad51(S208E-A209D)–Cas9(D10A)Expresses the hRad51(S208E-A209D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter
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125562hRad51(A190L-A192L)–Cas9(D10A)Expresses the Rad51(A190L-A192L)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter
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125563hRad51(A89E)–Cas9(D10A)Expresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter
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125564hRad51(F86E)–Cas9(D10A)Expresses the Rad51(F86E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter
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125565hRad51(G151D)–Cas9(D10A)Expresses the Rad51(G151D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter
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125567Rad51–Cas9(D10A)Expresses the Rad51–Cas9(D10A) fusion construct in mammalian cells under CMV promoter
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125568hRad51(K133R)–Cas9(D10A)Expresses the Rad51(K133R)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter
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125570CMV-hRad51Expresses the Rad51 protein in mammalian cells under CMV promoter
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125571hRad51(K133R)Expresses the hRad51(K133R) protein in mammalian cells under CMV promoter
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