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Engineering Introns to Express RNA Guides for Cas9- and Cpf1-Mediated Multiplex Genome Editing.
Ding D, Chen K, Chen Y, Li H, Xie K
Mol Plant. 2018 Apr 2;11(4):542-552. doi: 10.1016/j.molp.2018.02.005. Epub 2018 Feb 17. PubMed Article

Plasmids from Article

ID Plasmid Purpose
158151pRGEB33Construction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.
158152pRGEB34Construction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editing
158153p32FnConstruct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and LbCpf1, respectively. For plant genome editing.
158154p32LbConstruct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and FnCpf1, respectively. For plant genome editing.
158155p33FnExpression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.
158156p33LbExpression of crRNA array and LbCas12a using one Pol II promoter. For plant genome editing.

Antibodies from Article