Systematic perturbation of retroviral LTRs reveals widespread long-range effects on human gene regulation.
Fuentes DR, Swigut T, Wysocka J
Elife. 2018 Aug 2;7. pii: 35989. doi: 10.7554/eLife.35989.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 191269 | PiggyBac dCas9-VPR | piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP |
| 191314 | PiggyBac dCas9-KRAB | piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP |
| 191316 | LTR5HS S. pyogenes scaffold CARGO array | Contains an array of guide RNAs targeting LTR5Hs elements |
| 191317 | LTR5HS S. aureus scaffold CARGO array | Contains an array of guide RNAs targeting LTR5Hs elements |
| 191319 | nontarget S. pyogenes scaffold CARGO array | Contains an array of nontargeting guide RNAs |