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Rapid Assembly of gRNA Arrays via Modular Cloning in Yeast.
McCarty NS, Shaw WM, Ellis T, Ledesma-Amaro R
ACS Synth Biol. 2019 Apr 2. doi: 10.1021/acssynbio.9b00041.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
125752Guide-Generating VectorGuide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assembly
125754Destination VectorDestination vector for assembling gRNA fragments in the second round of assembly.