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Enzyme-Mediated Intercellular Proximity Labeling for Detecting Cell-Cell Interactions.
Ge Y, Chen L, Liu S, Zhao J, Zhang H, Chen PR
J Am Chem Soc. 2019 Feb 6;141(5):1833-1837. doi: 10.1021/jacs.8b10286. Epub 2019 Jan 29.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
125792pDisplay-mgSrtATransfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.
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125793pDisplay-ZHER-mgSrtAfor co-expression of ZHER (a HER2 binding affibody) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of HER2 positive cells.
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125794pcDNA3.1-CTLA4-mgSrtAfor co-expression of CTLA4 and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD80/86 positive cells.
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125795pcDNA3.1-CD40L-mgSrtAfor co-expression of CD40L (CD40 ligand) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD40 positive cells.
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125796pcDNA3.1-CD40L (lose of function mutant)-mgSrtAfor co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.
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