Addgene: Repurposing the Homing Endonuclease I-SceI for Positive Selection and Development of Gene-Editing Technologies.

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Kevin Solomon
Lee et al

Repurposing the Homing Endonuclease I-SceI for Positive Selection and Development of Gene-Editing Technologies.
Lee KZ, Mechikoff MA, Parasa MK, Rankin TJ, Pandolfi P, Fitzgerald KS, Hillman ET, Solomon KV
ACS Synth Biol. 2022 Jan 21;11(1):53-60. doi: 10.1021/acssynbio.1c00340. Epub 2022 Jan 10. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
179578	pColE1-ISceI	Expresses I-SceI from an arabinose promoter
179579	pFREE2-xCas9-sgRNA	Expresses xCas9 and gRNA that targets ColE1 plasmids for curing.
179580	pFREE2-eSpCas9-sgRNA	Expresses eSpCas9 and gRNA that targets ColE1 plasmids for curing
179582	pFREE2-SpCas9-sgRNA	Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

Plasmids from Article

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