Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion.
Menacho-Melgar R, Ye Z, Moreb EA, Yang T, Efromson JP, Decker JS, Wang R, Lynch MD
Biotechnol Bioeng. 2020 May 22. doi: 10.1002/bit.27440. PubMed Article
Menacho-Melgar R, Ye Z, Moreb EA, Yang T, Efromson JP, Decker JS, Wang R, Lynch MD
Biotechnol Bioeng. 2020 May 22. doi: 10.1002/bit.27440. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 65814 | pSMART-Ala1 | NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression |
| 65822 | pSMART-GFPuv | GFPuv Expression, Low Phosphate Dependent E. coli Expression |
| 87135 | pSMART-ala10 | NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression |
| 127058 | pHCKan-yibDp-mCherry | Low phosphate inducible mCherry with N-terminal linker and His tag |
| 127060 | pTKhc-yibDp-GFP-β20cp6 | Low phosphate inducible circular permutation of GFP, which is a substrate of the lon protease |
| 127078 | pHCKan-yibDp-GFPuv | Low phosphate inducible yibD gene promoter expressing GFPuv reporter |
| 134590 | pHCKan-yibDp-mdlC | Low Phosphate inducible mdlC benzylformate decarboxylase |
| 134592 | pHCKan-yibDp-GST | Low Phosphate inducible GST |
| 134593 | pHCKan-INSyibp-Nef | Low Phosphate inducible Nef |
| 134595 | pHCKan-yibDp-cimA3.7 | Low Phosphate inducible mutant citramalate synthase |
| 134596 | pHCKan-yibDp-CBD-hGLY | Low Phosphate inducible hGLYAT2 with N-terminal chitin binding domain tag |
| 134597 | pCDF-yibDp-matB | Low Phosphate inducible matB (malonyl-CoA synthetase) |
| 134598 | pHCKan-yibDp-SBS-mCherry | Low Phosphate inducible SBS (bonryl-diphosphate) synthase with C-terminal mCherry) |
| 134938 | pTKhc-yibDp-GFP-cp6 | Low phosphate inducible circular permutation of GFP |
| 134939 | pHCKan-yibDp-ald*-alaE | Low phosphate inducible alanine dehydrogenase and alanine exporter |
| 134940 | pHCKan-T7-CBD-hGLY | T7 based expression of human glycine acyltransferase with an N-terminal chitin binding domain |