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Excised linear introns regulate growth in yeast.
Morgan JT, Fink GR, Bartel DP
Nature. 2019 Jan;565(7741):606-611. doi: 10.1038/s41586-018-0828-1. Epub 2019 Jan 16.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
127596pRS416-ECM33_intronlow-copy URA-selectable plasmid, ECM33 intron inserted into URA3
127597pRS416-PTEF1-MCP-no_intronlow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter
127598pRS416-PTEF1-MCP-ECM33_intronlow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, ECM33 intron inserted into URA3
127599pRS416-PTEF1-MCP-5P-ECM33_intronlow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5P-ECM33 intron inserted into URA3
127600pRS416-PTEF1-MCP-5M-ECM33_intronlow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3
127601pRS416-PTEF1-MCP-3P-ECM33_intronlow-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 3P-ECM33 intron inserted into URA3
127602pRS416-ACT1_intronlow-copy URA-selectable plasmid, ACT1 intron inserted into URA3
127603pRS416-HNT2_intronlow-copy URA-selectable plasmid, HNT2 intron inserted into URA3
127604pRS416-RPL27B_intronlow-copy URA-selectable plasmid, RPL27B intron inserted into URA3
127605pRS416-APS3_intronlow-copy URA-selectable plasmid, APS3 intron inserted into URA3
127606pRS426-ECM_stablehigh-copy URA-selectable plasmid, WT ECM33 intron (stable) inserted into URA3
127607pRS426-ECM_unstablehigh-copy URA-selectable plasmid, Mut ECM33 intron (unstable) inserted into URA3
127608pRS426-ACT_unstablehigh-copy URA-selectable plasmid, WT ACT1 intron (unstable) inserted into URA3
127609pRS426-ACT_stablehigh-copy URA-selectable plasmid, Mut ACT1 intron (stable) inserted into URA3

Antibodies from Article