Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
rec-YnH enables simultaneous many-by-many detection of direct protein-protein and protein-RNA interactions.
Yang JS, Garriga-Canut M, Link N, Carolis C, Broadbent K, Beltran-Sastre V, Serrano L, Maurer SP
Nat Commun. 2018 Sep 14;9(1):3747. doi: 10.1038/s41467-018-06128-x. PubMed Article

Plasmids from Article

ID Plasmid Purpose
133893pAWExpression of Gal4AD-Prey hybrid protein
133894pAWHExpression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22H
133895pAWH-largeTPositive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pB
133896pAWH-IRPPositive control when used with pMS22H-IRE. Expression of Gal4AD-IRP hybrid protein. Homology regions for recombination with pMS22H
133897pBWExpression of Gal4BD-Bait hybrid protein.
133898pBKWHExpression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH
133899pBKWH-LamNegative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWH
133900pBKWH-p53Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH
133901pBSWHhcExpression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH
133902pBSWHhc-LamNegative control when used in combination with pAWH-largeT. Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH
133903pBSWHhc-p53Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH
133904pMS22HExpression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWH
133905pMS22H-IREPositive control when used in combination with pAWH-IRP. Expression of MS2BS-IRE site hybrid. Homology regions for recombination with pAWH
136403pHTNWExpression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.
136404pNLF1WExpression of N-terminal NanoLuc luciferase fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

Antibodies from Article