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rec-YnH enables simultaneous many-by-many detection of direct protein-protein and protein-RNA interactions.
Yang JS, Garriga-Canut M, Link N, Carolis C, Broadbent K, Beltran-Sastre V, Serrano L, Maurer SP
Nat Commun. 2018 Sep 14;9(1):3747. doi: 10.1038/s41467-018-06128-x.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
133893pAWExpression of Gal4AD-Prey hybrid protein
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133894pAWHExpression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22H
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133895pAWH-largeTPositive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pB
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133896pAWH-IRPPositive control when used with pMS22H-IRE. Expression of Gal4AD-IRP hybrid protein. Homology regions for recombination with pMS22H
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133897pBWExpression of Gal4BD-Bait hybrid protein.
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133898pBKWHExpression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH
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133899pBKWH-LamNegative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWH
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133900pBKWH-p53Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH
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133901pBSWHhcExpression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH
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133902pBSWHhc-LamNegative control when used in combination with pAWH-largeT. Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH
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133903pBSWHhc-p53Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH
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133904pMS22HExpression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWH
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133905pMS22H-IREPositive control when used in combination with pAWH-IRP. Expression of MS2BS-IRE site hybrid. Homology regions for recombination with pAWH
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136403pHTNWExpression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.
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136404pNLF1WExpression of N-terminal NanoLuc luciferase fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.
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Recombinant Antibodies from Article