Combining optogenetics with sensitive FRET imaging to monitor local microtubule manipulations.
Van Geel O, Cheung S, Gadella TWJ
Sci Rep. 2020 Apr 7;10(1):6034. doi: 10.1038/s41598-020-62874-3. PubMed Article
Van Geel O, Cheung S, Gadella TWJ
Sci Rep. 2020 Apr 7;10(1):6034. doi: 10.1038/s41598-020-62874-3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128002 | ROPY (sYFP2-OP18-mScI-R125I) | Improved redshifted FRET-readout of Stathmin/OP18 interaction with α-tubulin |
| 128003 | sYFP2-OP18-mScI | Redshifted FRET-readout of Stathmin/OP18 interaction with α-tubulin |
| 128004 | ROPY-2C2 | Local FRET-readout of membrane-anchored Stathmin/OP18 interaction with α-tubulin |
| 128005 | ROPY-4A | Unphosphorylatable mutant of Stathmin/OP18 FRET-sensor |
| 128006 | ROPY-4E | Phosphomimic mutant of Stathmin/OP18 FRET-sensor |
| 128007 | EB3-iRFP713 | In vivo visualization of microtubule plus ends with near-infrared |
| 128008 | ROPY-sYFP2(Y67G) | Dark sYFP2 mutant of ROPY |