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Tools for Mos1-mediated single copy insertion (mosSCI) with excisable unc-119(+) or NeoR (G418) selection cassettes.
Aram R, MacGillivray K, Li C, Saltzman A
MicroPubl Biol. 2019 Aug 27;2019. doi: 10.17912/micropub.biology.000146. PubMed Article

Plasmids from Article

ID Plasmid Purpose
129538pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)modified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selection
129539pALS186 (ttTi5605-MCS loxP::C.br.unc-119(+)::loxP)modified pCFJ151 for C. elegans mosSCI at ttTi5605 or compatible sites; floxed C. br. unc-119(+) selection

Antibodies from Article