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Single-Molecule Imaging Uncovers Rules Governing Nonsense-Mediated mRNA Decay.
Hoek TA, Khuperkar D, Lindeboom RGH, Sonneveld S, Verhagen BMP, Boersma S, Vermeulen M, Tanenbaum ME
Mol Cell. 2019 May 23. pii: S1097-2765(19)30361-2. doi: 10.1016/j.molcel.2019.05.008.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
130697TPI-WTExpresses TPI-Wildtype NMD reporter
130698TPI-PTC160Expresses TPI-PTC160 NMD reporter (It has a Premature termination codon (PTC) at 160th Amino acid)
130699TPI-PTC1Expresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid)
130700Beta-Globin-WTExpresses Beta-Globin WT NMD reporter
130701Beta-Globin-PTC39Expresses Beta-globin-PTC39 NMD reporter (It has a Premature termination codon (PTC) at 39th Amino acid)
131043HP21-TPI-PTC1Expresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid) with a 21 nt hairpin in 5'UTR
131044HP21-TPI-WT-no_intronsExpresses TPI-Wildtype NMD reporter without introns and with a 21 nt hairpin in 5'UTR

Antibodies from Article