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Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems.

Yang LZ, Wang Y, Li SQ, Yao RW, Luan PF, Wu H, Carmichael GG, Chen LL
Mol Cell. 2019 Dec 19;76(6):981-997.e7. doi: 10.1016/j.molcel.2019.10.024. Epub 2019 Nov 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
132397pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlagoverexpression in human cells
132398pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlagoverexpression in human cells
132399pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlagoverexpression in human cells
132400pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlagoverexpression in human cells
132401pHAGE-IRES-puro-NLS-dPspCas13b-mNeongreen-NLS-3xFlagoverexpression in human cells
132402pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlagoverexpression in human cells
132403pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlagoverexpression in human cells
132404pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLSoverexpression in human cells
132405pHAGE-NLS-dPspCas13b-3xmRuby3-NLSoverexpression in human cells
132406pHAGE-NLS-dPguCas13b-3xmRuby3-NLSoverexpression in human cells
132407pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlagoverexpression in human cells
132408pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlagoverexpression in human cells
132409pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlagoverexpression in human cells
132410pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlagoverexpression in human cells
132411pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlagoverexpression in human cells
132412pHAGE-IRES-puro-NLS-dAdmCas13d-EGFP-NLS-3xFlagoverexpression in human cells
132413pmRuby3-24xGCN4overexpression in human cells

Antibodies from Article