Purposeoverexpression in human cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||132404||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesPrevotella sp. P5-125
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Plasmid is prone to recombination. Test multiple colonies to ensure the full plasmid is isolated.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS was a gift from Ling-Ling Chen (Addgene plasmid # 132404 ; http://n2t.net/addgene:132404 ; RRID:Addgene_132404)
For your References section:Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems. Yang LZ, Wang Y, Li SQ, Yao RW, Luan PF, Wu H, Carmichael GG, Chen LL. Mol Cell. 2019 Dec 19;76(6):981-997.e7. doi: 10.1016/j.molcel.2019.10.024. Epub 2019 Nov 19. 10.1016/j.molcel.2019.10.024 PubMed 31757757