A transient reporter for editing enrichment (TREE) in human cells.
Standage-Beier K, Tekel SJ, Brookhouser N, Schwarz G, Nguyen T, Wang X, Brafman DA
Nucleic Acids Res. 2019 Aug 20. pii: 5552066. doi: 10.1093/nar/gkz713.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 138269 | pEF-BE4Gam | Expression of BE4am-Cas9 Base-editor from an EF1a promoter. |
| 138270 | pEF-AncBE4max | Expression of AncBE4max-Cas9 Base-editor from an EF1a promoter. |
| 138271 | pDT-sgRNA | Dual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS) |
| 138272 | pEF-BFP | Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor |