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Optimizing Rhizobium-legume symbioses by simultaneous measurement of rhizobial competitiveness and N2 fixation in nodules.
Mendoza-Suarez MA, Geddes BA, Sanchez-Canizares C, Ramirez-Gonzalez RH, Kirchhelle C, Jorrin B, Poole PS
Proc Natl Acad Sci U S A. 2020 May 5;117(18):9822-9831. doi: 10.1073/pnas.1921225117. Epub 2020 Apr 21.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
133123pOGG043Level 0 PU module synthesised as a fragment (pL0M-PU-PsNifH). Synthetic consensus promoter to drive nodule-specific expression in biovars and strains of R. leguminosarum.
133124pOGG113pL0M-PU-NoP, promotorless region used as negative control
133227pOPS0262Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
133228pOPS0263Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
133229pOPS0378Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
133230pOPS0380Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
133231pOPS0381Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
133232pOPS0382Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
133233pOPS0383Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
133234pOPS0384Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
133235pOPS0385Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.
133236pOPS0386Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

Antibodies from Article