Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Lysine acylation using conjugating enzymes for site-specific modification and ubiquitination of recombinant proteins.
Hofmann R, Akimoto G, Wucherpfennig TG, Zeymer C, Bode JW
Nat Chem. 2020 Nov;12(11):1008-1015. doi: 10.1038/s41557-020-0528-y. Epub 2020 Sep 14.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
133909pET28-His6-Ubc9Bacterial expression of His6-Ubc9
133910pET28-His6-Ubc9-C93ABacterial expression of His6-Ubc9-C93A
133911pET28-His6-Ubc9-C138ABacterial expression of His6-Ubc9-C138A
133913pET28-His6-GFP-C-LACEBacterial expression of His6-GFP-C-LACE

Antibodies from Article