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A PopZ-Linked Apical Recruitment Assay for Studying Protein-Protein Interactions in the Bacterial Cell Envelope.
Lim HC, Bernhardt TG
Mol Microbiol. 2019 Sep 24. doi: 10.1111/mmi.14391. PubMed Article

Plasmids from Article

ID Plasmid Purpose
134454pHCL147TetR, lacI-Plac-ssdsbA-mscarC, Integrative plasmid for the expression of fully-secreted prey. C-terminal mScarlet-I fusion.
134455pHCL151TetR, lacI-Plac-ssdsbA-mscarN, Integrative plasmid for the expression of fully-screted prey. N-terminal mScarlet-I fusion.
134456pHCL150CmR, Para-popZ-rbs-msfGFPC-H3H4. Replicative plasmid for the expression of bait. C-terminal GFP fusion.
134457pHCL149CmR, Para-popZ-rbs-H3H4-msfGFPN-CTM. Replicative plasmid for the expression of bait. N-terminal GFP fusion.

Antibodies from Article