Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair pathways.
Taghbalout A, Du M, Jillette N, Rosikiewicz W, Rath A, Heinen CD, Li S, Cheng AW
Nat Commun. 2019 Sep 20;10(1):4296. doi: 10.1038/s41467-019-12339-7. PubMed Article
Taghbalout A, Du M, Jillette N, Rosikiewicz W, Rath A, Heinen CD, Li S, Cheng AW
Nat Commun. 2019 Sep 20;10(1):4296. doi: 10.1038/s41467-019-12339-7. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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134632 | pAT243_pX-sgRNA-5xPBSa-5xPBSc | Cloning vector for expression of sgRNAs with dual PUF-Binding Sites (5xPBSa - 5xPBSc) of Casilio-ME 2.3, 2.4, 3.3 and 3.4 |
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134643 | pAT888_CMV/CAG_PUFa-hTET1(CD) | 3rd generation lentiviral expression of untagged Casilio-ME1 effector PUFa-hTET1(CD) in mammalian cells |
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134649 | pAT890_CMV/CAG_dCas9-hTET1(CD) | 3rd generation lentiviral plasmid for expression of Sp dCas9-hTET1(CD) fusion protein in mammalian cells |
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134650 | pAT801_CMV/CAG_TALE_A-hTET1(CD) | 3rd generation lentiviral expression of TALE-hTET1(CD) in mammalian cells targeting MLH1 promoter region |
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134740 | pAT812_CMV/CAG_TALE_B-hTET1(CD) | 3rd generation lentiviral expression of TALE-hTET1(CD) in mammalian cells targeting MLH1 promoter region |
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134829 | pAT806_CMV/CAG_TALE_D-hTET1(CD) | 3rd generation lentiviral expression of TALE-hTET1(CD) in mammalian cells targeting MLH1 promoter region |
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134830 | pAT817_CMV/CAG_TALE_F-hTET1(CD) | 3rd generation lentiviral expression of TALE-hTET1(CD) in mammalian cells targeting MLH1 promoter region |
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134831 | pAT341_CMV/CAG_PUFa-TET1CD (H1671Y D1673A) (dead TET1) | 3rd generation lentiviral expression of catalytically inactive Casilio-ME1 effector containing dead hTET1(CD) H1671Y D1673A in mammalian cells |
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134835 | pAT360_CMV/CAG_PUFa-hGADD45A-hTET1(CD) | 3rd generation lentiviral expression of untagged Casilio-ME2.1 effector PUFa-hGADD45A-hTET1(CD) in mammalian cells |
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134837 | pAT635_CMV/CAG_hGADD45A-PUFa-hTET1(CD) | 3rd generation lentiviral expression of untagged Casilio-ME2.2 effector hGADD45A-PUFa-hTET1(CD) in mammalian cells |
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134838 | pAT355_CMV/CAG_PUFc-hGADD45A | 3rd generation lentiviral expression of Casilio-ME2.3 effector PUFc-hGADD45A in mammalian cells |
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134846 | pAT356-CMV/CAG_hGADD45A-PUFc | 3rd generation lentiviral expression of Casilio-ME2.4 effector hGADD45A-PUFc in mammalian cells |
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134848 | pAT892_CMV/CAG_PUFa-p65HSF1 | 3rd generation lentiviral expression of Casilio activator PUFa-p65HSF1 in mammalian cells |
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134849 | pAT608_CMV/CAG_Flag-hNEIL2-PUFa-hTET1(CD) | 3rd generation lentiviral expression of Flag-tagged Casilio-ME3.1 effector hNEIL2-PUFa-hTET1(CD) in mammalian cells |
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134850 | pAT611_CMV/CAG_PUFa-hNEIL2-hTET1(CD) | 3rd generation lentiviral expression of Casilio-ME3.2 effector PUFa-hNEIL2-hTET1(CD) in mammalian cells |
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134851 | pAT595_CMV/CAG_PUFc-hNEIL2 | 3rd generation lentiviral expression of Casilio-ME3.3 effector PUFc-hNEIL2 in mammalian cells |
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134855 | pAT596_CMV/CAG_hNEIL2-PUFc | 3rd generation lentiviral expression of Casilio-ME3.4 effector hNEIL2-PUFc in mammalian cells |
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134856 | pAT699-CMV/CAG_Flag-hGADD45A-PUFa-hTET1(CD) | 3rd generation lentiviral expression of Flag-tagged Casilio-ME2.2 effector hGADD45A-PUFa-hTET1(CD) in mammalian cells |
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134870 | pAT976_CMV/CAG_Flag-hGADD45A-PUFa-dTET1CD | 3rd generation lentiviral expression of Flag-tagged and catalytically inactive Casilio-ME2.2 effector containing dead hTET1(CD) H1671Y D1673A in mammalian cells |
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134871 | pAT971_CMV/CAG_Flag-hGADD45A (G39A)-PUFa-hTET1(CD) | 3rd generation lentiviral expression of Flag-tagged Casilio-ME2.2 effector with G39A GADD45A mutation hGADD45A(G39A)-PUFa-hTET1(CD) in mammalian cells |
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134872 | pAT972_CMV/CAG_Flag-hGADD45A (L77E)-PUFa-hTET1(CD) | 3rd generation lentiviral expression of Flag-tagged Casilio-ME2.2 effector with L77E GADD45A mutation hGADD45A (L77E)-PUFa-hTET1(CD) in mammalian cells |
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134873 | pAT973_CMV/CAG_FlaghGADD45A (G39A, L77E)-PUFa-hTET1(CD) | 3rd generation lentiviral expression of Flag-tagged Casilio-ME2.2 effector with G39A L77E GADD45A mutations hGADD45A(G39A L77E)-PUFa-hTET1(CD) in mammalian cells |
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134875 | pAT977_CMV/CAG_Flag-hNEIL2-PUFa-dTET1(CD) | 3rd generation lentiviral expression of Flag-tagged Casilio-ME3.1 effector containing a catalytically dead hTET1(CD) in mammalian cells |
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134876 | pAT1059_CMV/CAG_Flag-hNEIL2 (C291S)-PUFa-hTET1(CD) | 3rd generation lentiviral expression of Flag-tagged Casilio-ME3.1 effector with C291S NEIL2 mutation hNEIL2(C291S)-PUFa-hTET1(CD) in mammalian cells |
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134877 | pAT1060_CMV/CAG_Flag-hNEIL2 (R310Q)-PUFa-hTET1(CD) | 3rd generation lentiviral expression of Flag-tagged Casilio-ME3.1 effector with R310Q NEIL2 mutation hNEIL2(R310-Q)-PUFa-hTET1(CD) in mammalian cells |
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134895 | pAT1089_PB-EF1a-Blast_2A_rtTA3-SV40pA_pCW-dCas9 | PiggyBac vector for establishement of doxycycline-inducible Sp dCas9 expression cell lines |
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134975 | pAT1501-[PB-4xHSab-EF1a-Hygro_2A_rtTA3-SV40pA(-)_pCW-DEST] | Destination vector for doxycycline-inducible expression from plasmid hosting piggyBac hygromycin cassettes |
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134976 | pAT1502-[PB-4xHSab-EF1a-Blast_2A_rtTA3-SV40pA(-)_pCW-DEST] | Destination vector for doxycycline-inducible expression from plasmid hosting piggyBac Blasticidin cassettes |
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134978 | pAT1503_pX-SPFE-BbsI(ccdbCam)-5xPBSa | Cloning vector for expression of Casilio sgRNAs containing five PUFa-binding sites (PBSa) |
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134979 | pAT1504_pX-SPFE-BbsI(ccdbCam)-5xPBSc | Cloning vector for expression of Casilio sgRNAs containing five PUFc-binding sites (PBSc) |
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