Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair pathways.
Taghbalout A, Du M, Jillette N, Rosikiewicz W, Rath A, Heinen CD, Li S, Cheng AW
Nat Commun. 2019 Sep 20;10(1):4296. doi: 10.1038/s41467-019-12339-7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
134632pAT243_pX-sgRNA-5xPBSa-5xPBScCloning vector for expression of sgRNAs with dual PUF-Binding Sites (5xPBSa - 5xPBSc) of Casilio-ME 2.3, 2.4, 3.3 and 3.4
Loading...
134643pAT888_CMV/CAG_PUFa-hTET1(CD)3rd generation lentiviral expression of untagged Casilio-ME1 effector PUFa-hTET1(CD) in mammalian cells
Loading...
134649pAT890_CMV/CAG_dCas9-hTET1(CD)3rd generation lentiviral plasmid for expression of Sp dCas9-hTET1(CD) fusion protein in mammalian cells
Loading...
134650pAT801_CMV/CAG_TALE_A-hTET1(CD)3rd generation lentiviral expression of TALE-hTET1(CD) in mammalian cells targeting MLH1 promoter region
Loading...
134740pAT812_CMV/CAG_TALE_B-hTET1(CD)3rd generation lentiviral expression of TALE-hTET1(CD) in mammalian cells targeting MLH1 promoter region
Loading...
134829pAT806_CMV/CAG_TALE_D-hTET1(CD)3rd generation lentiviral expression of TALE-hTET1(CD) in mammalian cells targeting MLH1 promoter region
Loading...
134830pAT817_CMV/CAG_TALE_F-hTET1(CD)3rd generation lentiviral expression of TALE-hTET1(CD) in mammalian cells targeting MLH1 promoter region
Loading...
134831pAT341_CMV/CAG_PUFa-TET1CD (H1671Y D1673A) (dead TET1)3rd generation lentiviral expression of catalytically inactive Casilio-ME1 effector containing dead hTET1(CD) H1671Y D1673A in mammalian cells
Loading...
134835pAT360_CMV/CAG_PUFa-hGADD45A-hTET1(CD)3rd generation lentiviral expression of untagged Casilio-ME2.1 effector PUFa-hGADD45A-hTET1(CD) in mammalian cells
Loading...
134837pAT635_CMV/CAG_hGADD45A-PUFa-hTET1(CD)3rd generation lentiviral expression of untagged Casilio-ME2.2 effector hGADD45A-PUFa-hTET1(CD) in mammalian cells
Loading...
134838pAT355_CMV/CAG_PUFc-hGADD45A3rd generation lentiviral expression of Casilio-ME2.3 effector PUFc-hGADD45A in mammalian cells
Loading...
134846pAT356-CMV/CAG_hGADD45A-PUFc3rd generation lentiviral expression of Casilio-ME2.4 effector hGADD45A-PUFc in mammalian cells
Loading...
134848pAT892_CMV/CAG_PUFa-p65HSF13rd generation lentiviral expression of Casilio activator PUFa-p65HSF1 in mammalian cells
Loading...
134849pAT608_CMV/CAG_Flag-hNEIL2-PUFa-hTET1(CD)3rd generation lentiviral expression of Flag-tagged Casilio-ME3.1 effector hNEIL2-PUFa-hTET1(CD) in mammalian cells
Loading...
134850pAT611_CMV/CAG_PUFa-hNEIL2-hTET1(CD)3rd generation lentiviral expression of Casilio-ME3.2 effector PUFa-hNEIL2-hTET1(CD) in mammalian cells
Loading...
134851pAT595_CMV/CAG_PUFc-hNEIL23rd generation lentiviral expression of Casilio-ME3.3 effector PUFc-hNEIL2 in mammalian cells
Loading...
134855pAT596_CMV/CAG_hNEIL2-PUFc3rd generation lentiviral expression of Casilio-ME3.4 effector hNEIL2-PUFc in mammalian cells
Loading...
134856pAT699-CMV/CAG_Flag-hGADD45A-PUFa-hTET1(CD)3rd generation lentiviral expression of Flag-tagged Casilio-ME2.2 effector hGADD45A-PUFa-hTET1(CD) in mammalian cells
Loading...
134870pAT976_CMV/CAG_Flag-hGADD45A-PUFa-dTET1CD3rd generation lentiviral expression of Flag-tagged and catalytically inactive Casilio-ME2.2 effector containing dead hTET1(CD) H1671Y D1673A in mammalian cells
Loading...
134871pAT971_CMV/CAG_Flag-hGADD45A (G39A)-PUFa-hTET1(CD)3rd generation lentiviral expression of Flag-tagged Casilio-ME2.2 effector with G39A GADD45A mutation hGADD45A(G39A)-PUFa-hTET1(CD) in mammalian cells
Loading...
134872pAT972_CMV/CAG_Flag-hGADD45A (L77E)-PUFa-hTET1(CD)3rd generation lentiviral expression of Flag-tagged Casilio-ME2.2 effector with L77E GADD45A mutation hGADD45A (L77E)-PUFa-hTET1(CD) in mammalian cells
Loading...
134873pAT973_CMV/CAG_FlaghGADD45A (G39A, L77E)-PUFa-hTET1(CD)3rd generation lentiviral expression of Flag-tagged Casilio-ME2.2 effector with G39A L77E GADD45A mutations hGADD45A(G39A L77E)-PUFa-hTET1(CD) in mammalian cells
Loading...
134875pAT977_CMV/CAG_Flag-hNEIL2-PUFa-dTET1(CD)3rd generation lentiviral expression of Flag-tagged Casilio-ME3.1 effector containing a catalytically dead hTET1(CD) in mammalian cells
Loading...
134876pAT1059_CMV/CAG_Flag-hNEIL2 (C291S)-PUFa-hTET1(CD)3rd generation lentiviral expression of Flag-tagged Casilio-ME3.1 effector with C291S NEIL2 mutation hNEIL2(C291S)-PUFa-hTET1(CD) in mammalian cells
Loading...
134877pAT1060_CMV/CAG_Flag-hNEIL2 (R310Q)-PUFa-hTET1(CD)3rd generation lentiviral expression of Flag-tagged Casilio-ME3.1 effector with R310Q NEIL2 mutation hNEIL2(R310-Q)-PUFa-hTET1(CD) in mammalian cells
Loading...
134895pAT1089_PB-EF1a-Blast_2A_rtTA3-SV40pA_pCW-dCas9PiggyBac vector for establishement of doxycycline-inducible Sp dCas9 expression cell lines
Loading...
134975pAT1501-[PB-4xHSab-EF1a-Hygro_2A_rtTA3-SV40pA(-)_pCW-DEST]Destination vector for doxycycline-inducible expression from plasmid hosting piggyBac hygromycin cassettes
Loading...
134976pAT1502-[PB-4xHSab-EF1a-Blast_2A_rtTA3-SV40pA(-)_pCW-DEST]Destination vector for doxycycline-inducible expression from plasmid hosting piggyBac Blasticidin cassettes
Loading...
134977pAT1090_PB-EF1a-Hygro_2A_rtTA3-SV40pA_pCW-Flag-PUFa-hTET1(CD)PiggyBac vector for establishement of doxycycline-inducible Casilio-ME1 effector expression cell lines
Loading...
134978pAT1503_pX-SPFE-BbsI(ccdbCam)-5xPBSaCloning vector for expression of Casilio sgRNAs containing five PUFa-binding sites (PBSa)
Loading...
134979pAT1504_pX-SPFE-BbsI(ccdbCam)-5xPBScCloning vector for expression of Casilio sgRNAs containing five PUFc-binding sites (PBSc)
Loading...