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Awakening dormant glycosyltransferases in CHO cells with CRISPRa.
Karottki KJC, Hefzi H, Xiong K, Shamie I, Hansen AH, Li S, Pedersen LE, Li S, Lee JS, Lee GM, Kildegaard HF, Lewis NE
Biotechnol Bioeng. 2020 Feb;117(2):593-598. doi: 10.1002/bit.27199. Epub 2019 Nov 12.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
134601VPR_dCas9Express CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 marker
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