Expression of Ca(2)(+)-permeable two-pore channels rescues NAADP signalling in TPC-deficient cells.
Ruas M, Davis LC, Chen CC, Morgan AJ, Chuang KT, Walseth TF, Grimm C, Garnham C, Powell T, Platt N, Platt FM, Biel M, Wahl-Schott C, Parrington J, Galione A
EMBO J. 2015 Jul 2;34(13):1743-58. doi: 10.15252/embj.201490009. Epub 2015 Apr 14. PubMed Article
Ruas M, Davis LC, Chen CC, Morgan AJ, Chuang KT, Walseth TF, Grimm C, Garnham C, Powell T, Platt N, Platt FM, Biel M, Wahl-Schott C, Parrington J, Galione A
EMBO J. 2015 Jul 2;34(13):1743-58. doi: 10.15252/embj.201490009. Epub 2015 Apr 14. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135182 | TPC1-mCherry | Expresses TPC1 with C-terminus mCherry tag in mammalian cells |
| 135183 | TPC2-mCherry | Expresses TPC2 with C-terminus mCherry tag in mammalian cells |
| 135184 | TPC1(ΔN69)-mCherry | TPC1 with truncated N-terminus, tagged with mCherry on C terminus. |
| 135185 | TPC2(ΔN49)-mCherry | TPC2 with truncated N-terminus, tagged with mCherry on C terminus. |
| 135186 | TPC2(N257A)-mCherry | Pore-dead mutant of TPC2, tagged on C terminus with mCherry. |
| 135187 | TPC2(E643A)-mCherry | Mutant TPC2 with reduced Ca2+ selectivity, tagged on C terminus with mCherry. |
| 135189 | TRPML1-mCherry | Expresses TRPML1 with C-terminus mCherry tag in mammalian cells. |