An electroporation-free method based on Red recombineering for markerless deletion and genomic replacement in the Escherichia coli DH1 genome.
Wei Y, Deng P, Mohsin A, Yang Y, Zhou H, Guo M, Fang H
PLoS One. 2017 Oct 24;12(10):e0186891. doi: 10.1371/journal.pone.0186891. eCollection 2017. PubMed Article
Wei Y, Deng P, Mohsin A, Yang Y, Zhou H, Guo M, Fang H
PLoS One. 2017 Oct 24;12(10):e0186891. doi: 10.1371/journal.pone.0186891. eCollection 2017. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135188 | pBDC | Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection. |
| 135190 | pBDK | Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection |
| 135191 | pCNA | Arabinose inducible expression of lamba-Red recombinase proteins and I-CreI. |
| 135192 | pSNA | Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette |
| 135193 | pSNK | Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassette |