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Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs.
Jost M, Santos DA, Saunders RA, Horlbeck MA, Hawkins JS, Scaria SM, Norman TM, Hussmann JA, Liem CR, Gross CA, Weissman JS
Nat Biotechnol. 2020 Jan 13. pii: 10.1038/s41587-019-0387-5. doi: 10.1038/s41587-019-0387-5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
136478Large-scale mismatched sgRNA library for gene titration (human essential genes, CRISPRi)Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi
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136479Compact mismatched sgRNA library for gene titration (human essential genes, CRISPRi)Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi
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Recombinant Antibodies from Article