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Split-miniSOG for Spatially Detecting Intracellular Protein-Protein Interactions by Correlated Light and Electron Microscopy.
Boassa D, Lemieux SP, Lev-Ram V, Hu J, Xiong Q, Phan S, Mackey M, Ramachandra R, Peace RE, Adams SR, Ellisman MH, Ngo JT
Cell Chem Biol. 2019 Oct 17;26(10):1407-1416.e5. doi: 10.1016/j.chembiol.2019.07.007. Epub 2019 Aug 1. PubMed Article

Plasmids from Article

ID Plasmid Purpose
136599pcDNA3-N-mSOG-bFos-IRES-mCherryEncodes bFos domain tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).
136600pcDNA3-bJun-C-mSOG-IRES-mCherryEncodes bJun domain tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).
136601pLV EF1A mSOG1-94-FRB-P2A-T2A-FKBP-mSOG-Ja95-140Lentiviral vector encoding mSOG1-94-FRB-P2A-T2A-FKBP-mSOG-Ja95-140 under the EF1A promoter.
136602pcDNA3-N-mSOG-FRB-IRES-mCherryEncodes FRB tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).
136603pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherryEncodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

Antibodies from Article