Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Continuous evolution of SpCas9 variants compatible with non-G PAMs.
Miller SM, Wang T, Randolph PB, Arbab M, Shen MW, Huang TP, Matuszek Z, Newby GA, Rees HA, Liu DR
Nat Biotechnol. 2020 Apr;38(4):471-481. doi: 10.1038/s41587-020-0412-8. Epub 2020 Feb 10.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
136918pCMV-BE4max-NRRHExpresses BE4max-NRRH in mammalian cells
136919pCMV-BE4max-NRTHExpresses BE4max-NRTH in mammalian cells
136920pCMV-BE4max-NRCHExpresses BE4max-NRCH in mammalian cells
136922pCMV-ABEmax-NRTHExpresses ABEmax-NRTH in mammalian cells
136923pCMV-ABEmax-NRCHExpresses ABEmax-NRCH in mammalian cells
136924pCMV-Cas9-NRRHExpresses Cas9-NRRH in mammalian cells
136925pCMV-Cas9-NRTHExpresses Cas9-NRTH in mammalian cells
136926pCMV-Cas9-NRCHExpresses Cas9-NRCH in mammalian cells
136927pTW211b5Directed evolution of SpCas9
136928pTW199b2Directed evolution of SpCas9
136929pTW170b2Directed evolution of SpCas9
136930pTW194b63Directed evolution of SpCas10
136931pTW195b63Directed evolution of SpCas11

Antibodies from Article