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DiCre-Based Inducible Disruption of Leishmania Genes.
Duncan SM, Myburgh E, Alves-Ferreira EV, Mottram JC
Methods Mol Biol. 2019;1971:211-224. doi: 10.1007/978-1-4939-9210-2_10.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
137047pGL2206Drug Resistance pDONR221SAT
137048pGL2207Drug Resistance pDONR221HYG
137049pGL2208Drug Resistance pDONR221BSD
137050pGL2209Drug Resistance pDONR221 PUR
137051pGL2399pRIB-DiCre DiCre expression from ribosomal locus
137053pGL2313Endogenous Locus DiCre Expression Cassette
137054pGL2314loxP (empty) expression cassette: c-terminal 6xHA
137055pGL2315loxP (empty) expression cassette: c-terminal GFP
137063pGL2316loxP (empty) expression cassette: n-terminal GFP

Antibodies from Article