iFLinkC: an iterative functional linker cloning strategy for the combinatorial assembly and recombination of linker peptides with functional domains.
Grawe A, Ranglack J, Weyrich A, Stein V
Nucleic Acids Res. 2020 Jan 11. pii: 5700546. doi: 10.1093/nar/gkz1210. PubMed Article
Grawe A, Ranglack J, Weyrich A, Stein V
Nucleic Acids Res. 2020 Jan 11. pii: 5700546. doi: 10.1093/nar/gkz1210. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 137090 | pL2-G | iFLinkC Linker cloning |
| 137091 | pL2-G2 | iFLinkC Linker cloning |
| 137092 | pL2-G3 | iFLinkC Linker cloning |
| 137093 | pL2-GPG | iFLinkC Linker cloning |
| 137094 | pL2-GPPPG | iFLinkC Linker cloning |
| 137095 | pL2-GGSG | iFLinkC Linker cloning |
| 137096 | pL2-GSPAG | iFLinkC Linker cloning |
| 137097 | pL2-(GGS)2G | iFLinkC Linker cloning |
| 137098 | pL2-(GGS)3G | iFLinkC Linker cloning |
| 137099 | pL2-(GGS)4GSG | iFLinkC Linker cloning |
| 137100 | pL2-G(P)7G | iFLinkC Linker cloning |
| 137101 | pL2-G(TP)4TG | iFLinkC Linker cloning |
| 137102 | pL2-GGASPAGG | iFLinkC Linker cloning |
| 137103 | pL2-GGASPAAPAPAG | iFLinkC Linker cloning |
| 137104 | pL2-GGA(EAAAK)2AGG | iFLinkC Linker cloning |
| 137105 | pL2-G(P)10G | iFLinkC Linker cloning |
| 137106 | pL2- GL(GGGS)3ETG | iFLinkC Linker cloning |
| 137107 | pFD-FRB | iFLinkC Linker cloning |
| 137108 | pFD-FKBP12 | iFLinkC Linker cloning |
| 137109 | pFD-NIa-Protease-TVMV | iFLinkC Linker cloning |
| 137110 | pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV) | iFLinkC Linker cloning |
| 137111 | pFD-AI(TVMV) | iFLinkC Linker cloning |
| 137112 | pFD-FN3 | iFLinkC Linker cloning |
| 137113 | pFD-ePDZ-B1 | iFLinkC Linker cloning |
| 137114 | pFLinkC-XE | iFLinkC Linker cloning |
| 137115 | pFLinkC-AI(GPG)-FKBP12(TP11)-FRB(GPG)-TVMV | Protease Switch Clone ID: 1-A9 |
| 137116 | pFLinkC-AI(GPG)-FKBP12(EAAAK16)-FRB(PAS5)-TVMV | Protease Switch Clone ID: 1-C9 |
| 137117 | pFLinkC-AI(G3)-PDZ(GPG-G)FN3(GGS4)-TVMV | Protease Switch Clone ID: 2-A5 |
| 137118 | pFLinkC-AI(GPG)-FKBP12(P9)-FRB(GPG)-TVMV | Protease Switch Clone ID: 2-B3 |