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A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies
Borden PM, Zhang P, Shivange AV, Marvin JS, Cichon J, Dan C, Podgorski K, Figueiredo A, Novak O, Tanimoto M, Shigetomi E, Lobas MA, Kim H, Zhu PK, Zhang Y, Zheng WS, Fan C, Wang G, Xiang B, Gan L, Zhang G, Guo K, Lin L, Cai Y, Yee AG, Aggarwal A, Ford CP, Rees DC, Dietrich D, Khakh BS, Dittman JS, Gan W, Koyama M, Jayaraman V, Cheer JF, Lester HA, Zhu JJ, Looger LL
bioRxiv 2020
Article

Plasmids from Article

ID Plasmid Purpose  
137950pAAV.hSynap.iAChSnFRExpresses iAChSnFR under hSynapsin promoter
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137951pAAV.hSynap.iAChSnFR-NULLExpresses iAChSnFR (non-binding) under hSynapsin promoter
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137953pAAV.hSynap.iAChSnFR-Venus-NULLExpresses iAChSnFR (non-binding yellow version) under hSynapsin promoter
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137955pAAV.CAG.iAChSnFRExpresses iAChSnFR under CAG promoter
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137956pAAV.CAG.iAChSnFR-NULLExpresses iAChSnFR (non-binding variant) under CAG promoter
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137959pAAV.CAG-FLEX.iAChSnFR-VenusExpresses FLEXed iAChSnFR (yellow version) under CAG promoter
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137960pAAV.CAG-FLEX.iAChSnFR-Venus-NULLExpresses FLEXed iAChSnFR (non-binding yellow version) under CAG promoter
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137962pAAV.ZAC.GFAP.iAChSnFRExpresses iAChSnFR under GFAP promoter
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140643pSinREP5-iAchSnFRAcetylcholine sensor for sindbis virus
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140644pLenti-hSynapsin-iAchSnFRAcetylcholine sensor for lenti virus
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Antibodies from Article