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Myc and Dnmt1 impede the pluripotent to totipotent state transition in embryonic stem cells.
Fu X, Wu X, Djekidel MN, Zhang Y
Nat Cell Biol. 2019 Jul;21(7):835-844. doi: 10.1038/s41556-019-0343-0. Epub 2019 Jun 17.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
138320pCW57-MCS1-P2A-MCS2-mDuxDox-inducible codon-optimized mouse Dux
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138324lentiCRISPR v2-sgMyc_1Express guide RNA 2 for mouse Myc
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138346lentiCRISPR v2-sgMyc_2Express guide RNA 1 for mouse Myc
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138347lentiCRISPR v2-sgDnmt1_1Express guide RNA 1 for Dnmt1
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138348lentiCRISPR v2-sgDnmt1_2Express guide RNA 2 for mouse Dnmt1
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