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Broadening the spectrum of fluorescent protein tools for use in the encapsulated human fungal pathogen Cryptococcus neoformans.
Spencer GWK, Chua SMH, Erpf PE, Wizrah MSI, Dyba TG, Condon ND, Fraser JA
Fungal Genet Biol. 2020 May;138:103365. doi: 10.1016/j.fgb.2020.103365. Epub 2020 Mar 4. PubMed Article

Plasmids from Article

ID Plasmid Purpose
139410pGWKS2Fluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised GFP S65T marker.
139411pGWKS4Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mCherry marker.
139412pGWKS5Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Citrine marker.
139413pGWKS6Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mMaroon1 marker.
139414pGWKS7Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mRuby3 marker.
139415pGWKS8Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTFP1 marker.
139416pGWKS9Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTurquoise2 marker.
139417pGWKS10Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Clover marker.
139418pGWKS11Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mNeonGreen marker.
139419pGWKS12Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Superfolder GFP marker.
139420pGWKS17Fluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised mCherry marker.
139421pPEE42Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the Citrine ORF and a flexible linker at the 5' end.
139422pPEE43Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mMaroon1 ORF and a flexible linker at the 5' end.
139423pPEE44Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTFP1 ORF and a flexible linker at the 5' end.
139424pPEE45Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTurquoise2 ORF and a flexible linker at the 5' end.
139425pPEE46Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.
139426pPEE47Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.

Antibodies from Article