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Minoru Ko Lab: Minoru Ko Lab plasmids
Unpublished

Plasmids from Article

ID Plasmid Purpose  
139517pDEST-flox-R4R3Provides two loxP sequences between gene cassettes in the destination vector
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139518pDEST-PB-R4R3Provides two PiggyBac transposon sequences between gene cassettes in the destination vector
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139519pDEST-EB-R4R3Provides episomal replication of the destination vector in primate cells
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139520pDEST-EB-Hyg-R4R3Provides episomal replication of the destination vector in primate cells
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139521pDEST-R4R3-MC1DTAProvides negative selection cassette on the vector
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139522pENTR-L4R1-CAGProvides constitutive promoter between attL4-attR1 cassette on Gateway entry vector
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139523pENTR-L4R1-hEF1aProvides constitutive promoter cassette between attL4-attR1 on Gateway entry vector
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139524pENTR-L4R1-PGKProvides constitutive promoter cassette between attL4-attR1 on Gateway entry vector
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139525pENTR-L4R1-tetO2Provides conditional promoter cassette between attL4-attR1 on Gateway entry vector
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139526pENTR-L4R1-PHtetO2Provides conditional promoter cassette and PGK promoter driven puromycin resistant gene cassette between attL4-attR1 on Gateway entry vector
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139527pENTR-L4R1-TREXProvides conditional promoter cassette between attL4-attR1 on Gateway entry vector
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139528pENTR-L4R1-hCMVstar1+bgloIntProvides conditional promoter cassette between attL4-attR1 on Gateway entry vector
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139529pENTR-R2L3-IRES-puro-pAProvides IRES-puro-pA cassette on Gateway entry vector
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139530pENTR-R2L3-IRES-Zeo-pAProvides IRES-Zeo-pA cassette on Gateway entry vector
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139533pENTR-R2L3-IRES-bgeo-pAProvides IRES-bgeo-pA cassette on Gateway entry vector
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139534pENTR-R2L3-IRES-BSD-pAProvides IRES-BSD-pA cassette on Gateway entry vector
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139535pENTR-R2L3-IRESpacDeltaTKpAProvides IRES-pacDeltaTK-pA cassette on Gateway entry vector
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139537pENTR-R2L3-cHA-IRESpuro-pAProvides HA protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector
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139538pENTR-R2L3-cGR-IRESpuro-pAProvides GR protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector
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139539pENTR-R2L3-cmER-IRESpuro-pAProvides mouse ER protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector
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139541pENTR-R2L3-cGR-IDProvides GR protein tag N-terminal to IRES-HisD-pA cassette on Gateway entry vector
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139542pENTR-R2L3-IRES-Venus-pAProvides IRES-Venus-pA cassette on Gateway entry vector
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139543pENTR-R2L3-pA-floxPGKneopAProvides pA sequences N-terminal to floxed PGK-neo-pA cassette on Gateway entry vector
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139544pENTR-R2L3-pA-PGKpacDeltaTK-pA1Provides pA sequences N-terminal to PGK-PacDeltaTK-pA cassette on Gateway entry vector
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139545pENTR-CreCre ORF with stop codon between attL1-attL2 on Gateway entry vector
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139546pENTR-CreStopDeletedCre ORF without stop codon between attL1-attL2 on Gateway entry vector
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139547pENTR-CreERT2CreERT2 ORF with stop codon between attL1-attL2 on Gateway entry vector
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139548pENTR-Flippase-mycFlippase-myc fusion ORF with stop codon between attL1-attL2 on Gateway entry vector
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139549pENTR-EmeraldEmerald ORF with stop codon between attL1-attL2 on Gateway entry vector
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139550pENTR-DsRedT4DsRedT4 ORF with stop codon between attL1-attL2 on Gateway entry vector
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139551pENTR-VenusVenus ORF with stop codon between attL1-attL2 on Gateway entry vector
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139552pENTR-d4Venusd4Venus ORF with stop codon between attL1-attL2 on Gateway entry vector
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139553pENTR-mKO1mKO1 ORF with stop codon between attL1-attL2 on Gateway entry vector
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139554pENTR_mCherrymCherry ORF with stop codon between attL1-attL2 on Gateway entry vector
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139555pENTR-HygroHygromycin resistant gene ORF with stop codon between attL1-attL2 on Gateway entry vector
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139556pENTR-rtTA3GrtTA ORF with stop codon between attL1-attL2 on Gateway entry vector
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139557pENTR-tTAF2tTAF2 ORF with stop codon between attL1-attL2 on Gateway entry vector
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139558pENTR-TetRTetR ORF with stop codon between attL1-attL2 on Gateway entry vector
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139559pENTR-L1R5-floxHygpAfloxed Hygromycin resistant gene with stop codon franking to pA sequeneces between attL1-attR5 on Gateway entry vector
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139560pENTR-L5L2-mCherry-stopmCherry ORF with stop codon between attL5-attL2 on Gateway entry vector
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139561PB-TET-PH-lox66FRTpiggyBac expression vector with TET-ON system and puromycin selection
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139562PB-TET-BH-lox66FRTpiggyBac expression vector with TET-ON system and Blasticidin selection
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139563PB-TET-ZH-lox66FRTpiggyBac expression vector with TET-ON system and Zeocin selection
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