LetB Structure Reveals a Tunnel for Lipid Transport across the Bacterial Envelope.
Isom GL, Coudray N, MacRae MR, McManus CT, Ekiert DC, Bhabha G
Cell. 2020 Apr 30;181(3):653-664.e19. doi: 10.1016/j.cell.2020.03.030. PubMed Article
Isom GL, Coudray N, MacRae MR, McManus CT, Ekiert DC, Bhabha G
Cell. 2020 Apr 30;181(3):653-664.e19. doi: 10.1016/j.cell.2020.03.030. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 139874 | pBEL1324 | Expression plasmid for WT LetB (without TM region) for cryo and negative stain EM |
| 139875 | pBEL1584 | Expression plasmid of rings 2 and 3 of LetB for crystallography |
| 139876 | pBEL1751 | Expression plasmid for naturally occurring 4xMCE protein (without TM region) |
| 139877 | pBEL1752 | Expression plasmid for naturally occurring 5xMCE protein (without TM region) |
| 139878 | pBEL1753 | Expression plasmid for naturally occurring 6xMCE protein (without TM region) |
| 139879 | pBEL1755 | Expression plasmid for naturally occurring 8xMCE protein (without TM region) |
| 139880 | pBEL2005 | Complementation plasmid for LetAB with 2 ring (rings17) LetB mutant |
| 139881 | pBEL2006 | Complementation plasmid for LetAB with 3 ring (rings 167) LetB mutant |
| 139882 | pBEL2007 | Complementation plasmid for LetAB with 4 ring (rings 1567) LetB mutant |
| 139883 | pBEL2008 | Complementation plasmid for LetAB with 5 ring (rings 12347) LetB mutant |
| 139884 | pBEL2009 | Complementation plasmid for LetAB with 6 ring (rings 123457) LetB mutant |
| 139885 | pBEL2000 | Expression plasmid for LetAB with 2 ring (rings17) LetB (without TM region) mutant |
| 139886 | pBEL2001 | Expression plasmid for LetAB with 3 ring (rings 167) LetB (without TM region) mutant |
| 139887 | pBEL2002 | Expression plasmid for LetAB with 4 ring (rings 1567) LetB (without TM region) mutant |
| 139888 | pBEL2003 | Expression plasmid for LetAB with 5 ring (rings 12347) LetB (without TM region) mutant |
| 139889 | pBEL2004 | Expression plasmid for LetAB with 6 ring (rings 123457) LetB (without TM region) mutant |
| 139890 | pBEL1886 | Complementation plasmid for LetAB with LetB PLL1 deletion |
| 139891 | pBEL1887 | Complementation plasmid for LetAB with LetB PLL2 deletion |
| 139892 | pBEL1888 | Complementation plasmid for LetAB with LetB PLL3 deletion |
| 139893 | pBEL1889 | Complementation plasmid for LetAB with LetB PLL4 deletion |
| 139894 | pBEL1890 | Complementation plasmid for LetAB with LetB PLL5 deletion |
| 139895 | pBEL1891 | Complementation plasmid for LetAB with LetB PLL6 deletion |
| 139896 | pBEL1892 | Complementation plasmid for LetAB with LetB PLL7 deletion |
| 139897 | pBEL1993 | Expression plasmid for LetB PLL1 deletion (without TM region) |
| 139898 | pBEL1994 | Expression plasmid for LetB PLL2 deletion (without TM region) |
| 139899 | pBEL1995 | Expression plasmid for LetB PLL3 deletion (without TM region) |
| 139900 | pBEL1996 | Expression plasmid for LetB PLL4 deletion (without TM region) |
| 139901 | pBEL1997 | Expression plasmid for LetB PLL5 deletion (without TM region) |
| 139902 | pBEL1998 | Expression plasmid for LetB PLL6 deletion (without TM region) |
| 139903 | pBEL1999 | Expression plasmid for LetB PLL7 deletion (without TM region) |
| 139904 | pBEL1857 | Complementation plasmid for LetAB with LetB mutant L243N |
| 139905 | pBEL1858 | Complementation plasmid for LetAB with LetB mutant L246N |
| 139906 | pBEL1859 | Complementation plasmid for LetAB with LetB mutant V247N |
| 139907 | pBEL1860 | Complementation plasmid for LetAB with LetB mutant L355N |
| 139908 | pBEL1861 | Complementation plasmid for LetAB with LetB mutant L358N |
| 139909 | pBEL1862 | Complementation plasmid for LetAB with LetB mutant L359N |
| 139910 | pBEL1806 | Expression plasmid for LetB mutant L243N |
| 139911 | pBEL1807 | Expression plasmid for LetB mutant L246N |
| 139912 | pBEL1808 | Expression plasmid for LetB mutant V247N |
| 139913 | pBEL1809 | Expression plasmid for LetB mutant L355N |
| 139914 | pBEL1810 | Expression plasmid for LetB mutant L358N |
| 139915 | pBEL1811 | Expression plasmid for LetB mutant L358N |
| 139916 | pBEL1725 | Expression plasmid for LetB mutant F468Bpa |
| 139917 | pBEL1726 | Expression plasmid for LetB mutant W476pa |
| 139918 | pBEL1727 | Expression plasmid for LetB mutant Y814Bpa |
| 139919 | pBEL1728 | Expression plasmid for LetB mutant F818Bpa |
| 139920 | pBEL1729 | Expression plasmid for LetB mutant F833Bpa |
| 139921 | pBEL1730 | Expression plasmid for LetB mutant K488Bpa |
| 139922 | pBEL1731 | Expression plasmid for LetB mutant E854Bpa |
| 139923 | pBEL2044 | Expression plasmid for 4 ring (rings 1237) LetB (without TM region) mutant |
| 139924 | pBEL2045 | Expression plasmid for 4 ring (rings 1347) LetB (without TM region) mutant |
| 139926 | pBEL2041 | Complementation plasmid for LetAB with 4 ring (rings 1237) LetB mutant |
| 139927 | pBEL2042 | Complementation plasmid for LetAB with 4 ring (rings 1347) LetB mutant |
| 139928 | pBEL2071 | Complementation plasmid for LetAB operon with LetA and LetB genes deoverlapped |
| 139929 | pBEL2039 | Complementation plasmid for LetAB without LetB TM region |
| 139930 | pBEL2040 | Complementation plasmid for LetAB with LetB TM region replaced by PelB leader sequence |