A novel family of expression vectors with multiple affinity tags for wheat germ cell-free protein expression.
Nagy SK, Kallai BM, Andras J, Meszaros T
BMC Biotechnol. 2020 Mar 14;20(1):17. doi: 10.1186/s12896-020-00610-5. PubMed Article
Nagy SK, Kallai BM, Andras J, Meszaros T
BMC Biotechnol. 2020 Mar 14;20(1):17. doi: 10.1186/s12896-020-00610-5. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140181 | pEU3-NII-HLICNot | Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 6x His-tag and NotI restriction site |
| 140182 | pEU3-NII-GLICNot | Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, GST-tag and NotI site |
| 140183 | pEU3-NII-HxHLICNot | Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 12xHis-tag and NotI restriction site |
| 140184 | pEU3-NII-FLAGLICNot | Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, FLAG-tag and NotI restriction site |
| 140185 | pEU3-NII-HaloLICNot | Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, Halo-tag and NotI restriction site |
| 140186 | pEU3-NII-GLICNot-C-BIOT | Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, GST-tag and biotinylation site, and NotI restriction site |
| 140187 | pEU3-NII-GLICNot-C-His | Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, and NotI restriction site |