Standard screening methods underreport AAV-mediated transduction and gene editing.
Lang JF, Toulmin SA, Brida KL, Eisenlohr LC, Davidson BL
Nat Commun. 2019 Jul 30;10(1):3415. doi: 10.1038/s41467-019-11321-7. PubMed Article
Lang JF, Toulmin SA, Brida KL, Eisenlohr LC, Davidson BL
Nat Commun. 2019 Jul 30;10(1):3415. doi: 10.1038/s41467-019-11321-7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174703 | pFB_ROSA26_SpCas9_gRNAs | Plasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation. |