Development of a genetic toolset for the highly engineerable and metabolically versatile Acinetobacter baylyi ADP1.
Biggs BW, Bedore SR, Arvay E, Huang S, Subramanian H, McIntyre EA, Duscent-Maitland CV, Neidle EL, Tyo KEJ
Nucleic Acids Res. 2020 Apr 4. pii: 5815822. doi: 10.1093/nar/gkaa167. PubMed Article
Biggs BW, Bedore SR, Arvay E, Huang S, Subramanian H, McIntyre EA, Duscent-Maitland CV, Neidle EL, Tyo KEJ
Nucleic Acids Res. 2020 Apr 4. pii: 5815822. doi: 10.1093/nar/gkaa167. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140634 | pBWB162 | pBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1 |
| 140636 | pBWB294 | pBAV1k-lacI-Trc-BCD9-mCherry. BCD RBS. |
| 140637 | pBWB419 | RSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus. |
| 140638 | pBWB424 | RSF1010-Cas9-vanAB gRNA. Vector with constitutive Cas9 and gRNA targeting vanAB locus. |