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Preloading budding yeast with all-in-one CRISPR/Cas9 vectors for easy and high-efficient genome editing.
Degreif D, Kremenovic M, Geiger T, Bertl A
J Biol Methods. 2018 Sep 12;5(3):e98. doi: 10.14440/jbm.2018.254. eCollection 2018.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
141249pCAS9iGalactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
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141250pCAS9cConstitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
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141251pCAS9idGalactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
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141252pCAS9cdConstitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
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