Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated gene silencing.
Huntzinger E, Braun JE, Heimstädt S, Zekri L, Izaurralde E
EMBO J. 2010 Dec 15;29(24):4146-60. doi: 10.1038/emboj.2010.274 PubMed Article
Huntzinger E, Braun JE, Heimstädt S, Zekri L, Izaurralde E
EMBO J. 2010 Dec 15;29(24):4146-60. doi: 10.1038/emboj.2010.274 PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 146543 | pCIneo-lambdaN-HA-HsTNRC6Biso1-siRNAres_I | Mammalian Expression of HsTNRC6Biso1-siRNAres |
| 146643 | pETM-41-DmPABPC1_J | Bacterial Expression of DmPABPC1 |
| 146644 | pETM-41-HsPABPC1_J | Bacterial Expression of HsPABPc1 |
| 146741 | pCIneo-lambdaN-HA-HsTNRC6A_1-1208-siRNAres_K | Mammalian Expression of HsTNRC6A_1-1208-siRNAres |
| 146742 | pCIneo-lambdaN-HA-HsTNRC6A_1242-1709-F1359A_K | Mammalian Expression of HsTNRC6A_1242-1709-F1359A |
| 146743 | pCIneo-lambdaN-HA-HsTNRC6A-del1352-1369-siRNAres_K | Mammalian Expression of HsTNRC6A-del1352-1369-siRNAres |
| 146744 | pCIneo-lambdaN-HA-HsTNRC6A-F1359A-siRNAres_K | Mammalian Expression of HsTNRC6A-F1359A-siRNAres |
| 146745 | pCIneo-lambdaN-HA-HsTNRC6Biso1_1250-1723-F1370A_K | Mammalian Expression of HsTNRC6Biso1_1250-1723-F1370A |
| 146746 | pCIneo-lambdaN-HA-HsTNRC6C_1248-1690-F1389A_K | Mammalian Expression of HsTNRC6C_1248-1690-F1389A |
| 146747 | pCIneo-lambdaN-HA-HsTNRC6C-F1389A_K | Mammalian Expression of HsTNRC6C-F1389A |
| 146820 | pAc5.1B-EGFP-DmGW182_1-1199-del862-1115-dsRNAres_L | Insect Expression of DmGW182_1-1199-del862-1115-dsRNAres |
| 146821 | pAc5.1B-EGFP-DmGW182_1-1199-delDUF-M2-dsRNAres_L | Insect Expression of DmGW182_1-1199-delDUF-M2-dsRNAres |
| 146822 | pAc5.1B-EGFP-DmGW182_1-1199-dsRNAres_L | Insect Expression of DmGW182_1-1199-dsRNAres |
| 146823 | pAc5.1B-EGFP-DmGW182_862-1384_L | Insect Expression of DmGW182_862-1384 |
| 146824 | pAc5.1B-EGFP-DmGW182_862-1384-F961A_L | Insect Expression of DmGW182_862-1384-F961A |
| 146825 | pAc5.1B-EGFP-DmGW182_1200-1384_L | Insect Expression of DmGW182_1200-1384 |
| 146826 | pAc5.1B-EGFP-DmGW182-AvrII-dsRNAres_L | Insect Expression of DmGW182-dsRNAres |
| 146827 | pAc5.1B-EGFP-DmGW182-delCterm-delDUF-dsRNAres_L | Insect Expression of DmGW182-delCterm-delDUF-dsRNAres |
| 146828 | pAc5.1B-EGFP-DmGW182-delCterm-delM2-dsRNAres_L | Insect Expression of DmGW182-delCterm-delM2-dsRNAres |
| 146829 | pAc5.1B-EGFP-DmGW182-delDUF-dsRNAres_L | Insect Expression of DmGW182-delDUF-dsRNAres |
| 146830 | pAc5.1B-EGFP-DmGW182-delDUF-M2-dsRNAres_L | Insect Expression of DmGW182-delDUF-M2-dsRNAres |
| 146831 | pAc5.1B-EGFP-DmGW182-dsRNAres_L | Insect Expression of DmGW182-dsRNAres |
| 146832 | pAc5.1B-EGFP-DmGW182-DUF-Cterm_L | Insect Expression of DmGW182-DUF-Cterm |
| 146833 | pAc5.1B-EGFP-DmGW182-DUF-RBD_L | Insect Expression of DmGW182-DUF-RBD |
| 146834 | pAc5.1B-EGFP-DmGW182-E962Q-dsRNAres_L | Insect Expression of DmGW182-E962Q-dsRNAres |
| 146835 | pAc5.1B-EGFP-DmGW182-F961A-dsRNAres_L | Insect Expression of DmGW182-F961A-dsRNAres |
| 146836 | pAc5.1B-EGFP-DmGW182-M2-RBD_L | Insect Expression of DmGW182-M2-RBD |
| 146837 | pAc5.1B-EGFP-DmGW182-PAM26B-dsRNAres_L | Insect Expression of DmGW182-PAM26B-dsRNAres |
| 146838 | pAc5.1B-EGFP-DmGW182-PAM26B-F1370A-dsRNAres_L | Insect Expression of DmGW182-PAM26B-F1370A-dsRNAres |
| 146839 | pAc5.1B-EGFP-DmGW182-RBD_L | Insect Expression of DmGW182-RBD |
| 146840 | pAc5.1B-EGFP-DmGW182-SD6B-dsRNAres_L | Insect Expression of DmGW182-SD6B-dsRNAres |
| 146841 | pAc5.1B-EGFP-DmGW182-SD6B-F13170A-dsRNAres_L | Insect Expression of DmGW182-SD6B-F1370A-dsRNAres |
| 146842 | pAc5.1B-EGFP-DmGW182-V958PQ959P-dsRNAres_L | Insect Expression of DmGW182-V958PQ959P-dsRNAres |
| 146843 | pAc5.1B-EGFP-HsTNRC6A_L | Insect Expression of HsTNRC6A |
| 146844 | pAc5.1B-EGFP-HsTNRC6A_1208-1709_L | Insect Expression of HsTNRC6A_1208-1709 |
| 146845 | pAc5.1B-EGFP-HsTNRC6A_1208-1709-F1359A_L | Insect Expression of HsTNRC6A_1208-1709-F1359A |
| 146846 | pAc5.1B-EGFP-HsTNRC6A-F1359A_L | Insect Expression of HsTNRC6A-F1359A |
| 146847 | pAc5.1B-EGFP-HsTNRC6Biso1_L | Insect Expression of HsTNRC6Biso1 |
| 146848 | pAc5.1B-EGFP-HsTNRC6Biso1_1-1619_L | Insect Expression of HsTNRC6Biso1_1-1619 |
| 146849 | pAc5.1B-EGFP-HsTNRC6Biso1_1-1619-del1395-1534_L | Insect Expression of HsTNRC6Biso1_1-1619-del1395-1534 |
| 146850 | pAc5.1B-EGFP-HsTNRC6Biso1_1218-1723_L | Insect Expression of HsTNRC6Biso1_1218-1723 |
| 146851 | pAc5.1B-EGFP-HsTNRC6Biso1_1218-1723-F1370A_L | Insect Expression of HsTNRC6Biso1_1218-1723-F1370A |
| 146852 | pAc5.1B-EGFP-HsTNRC6Biso1-del1359-1393_L | Insect Expression of HsTNRC6Biso1-del1359-1393 |
| 146853 | pAc5.1B-EGFP-HsTNRC6Biso1-del1395-1534_L | Insect Expression of HsTNRC6Biso1-del1395-1534 |
| 146854 | pAc5.1B-EGFP-HsTNRC6Biso1-F1370A_L | Insect Expression of HsTNRC6Biso1-F1370A |
| 146855 | pAc5.1B-EGFP-HsTNRC6C_L | Insect Expression of HsTNRC6C |
| 146856 | pAc5.1B-EGFP-HsTNRC6C_1207-1690_L | Insect Expression of HsTNRC6C_1207-1690 |
| 146857 | pAc5.1B-EGFP-HsTNRC6C_1207-1690-F1389A_L | Insect Expression of HsTNRC6C_1207-1690-F1389A |
| 146858 | pAc5.1B-EGFP-HsTNRC6C-F1389A_L | Insect Expression of HsTNRC6C-F1389A |
| 146859 | pAc5.1B-lambdaN-DmPABPC1_1-550-siRNAres_L | Insect Expression of DmPABPC1_1-550-siRNAres |
| 146860 | pAc5.1B-lambdaN-DmPABPC1_90-635-siRNAres-V5His6_L | Insect Expression of DmPABPC1_90-635-siRNAres |
| 146861 | pAc5.1B-lambdaN-DmPABPC1-del91-164-V5His6_L | Insect Expression of DmPABPC1_del91-164 |
| 146862 | pAc5.1B-lambdaN-DmPABPC1-del183-258-siRNAres-V5His6_L | Insect Expression of DmPABPC1_del183-258-siRNAres |
| 146863 | pAc5.1B-lambdaN-DmPABPC1-del288-359-siRNAres-V5His6_L | Insect Expression of DmPABPC1_del288-359-siRNAres |
| 146864 | pAc5.1B-lambdaN-DmPABPC1-del364-550-siRNAres-V5His6_L | Insect Expression of DmPABPC1_del364-550-siRNAres |
| 146865 | pEGFP-C1-HsTNRC6C_L | Mammalian Expression of HsTNRC6C |
| 146866 | pETM-41-DmPABPC1_1-390_L | Bacterial Expression of DmPABPC1_1-390 |
| 146867 | pETM-41-HsPABPC1_1-398_L | Bacterial Expression of HsPABPC1_1-398 |
| 146868 | pGEX-6P-1-DmGW182_862-1199_L | Bacterial Expression of DmGW182_862-1199 |
| 146869 | pGEX-6P-1-DmGW182_862-1199-del1004-1115_L | Bacterial Expression of DmGW182_862-1199-del1004-1115 |
| 146870 | pGEX-6P-1-DmGW182_862-1384_L | Bacterial Expression of DmGW182_862-1384 |
| 146872 | pGEX-6P-1-DmGW182_862-1384-del1004-1115_L | Bacterial Expression of DmGW182_862-1384-del1004-1115 |
| 146873 | pGEX-6P-1-HsTNRC6Biso1_1219-1621_L | Bacterial Expression of HsTNRC6Biso1_1219-1621 |
| 146874 | pGEX-6P-1-HsTNRC6Biso1_1219-1621-del1395-1534_L | Bacterial Expression of HsTNRC6Biso1_1219-1621-del1395-1534 |
| 146875 | pGEX-6P-1-HsTNRC6Biso1_1219-1723-del1359-1393_L | Bacterial Expression of HsTNRC6Biso1_1219-1723-del1359-1393 |
| 146876 | pGEX-6P-1-HsTNRC6Biso1_1219-1723-del1395-1534_L | Bacterial Expression of HsTNRC6Biso1_1219-1723-del1395-1534 |
| 146877 | pGEX-6P-1-HsTNRC6Biso1_1361-1723-6His_L | Bacterial Expression of HsTNRC6Biso1_1361-1723 |
| 146878 | pGEX-6P-1-HsTNRC6Biso1_1379-1723_L | Bacterial Expression of HsTNRC6Biso1_1379-1723 |
| 147024 | pCIneo-lambdaN-HA-HsTNRC6A_1-1609-del1384-1524-siRNAres_N | Mammalian Expression of HsTNRC6A_1-1609-del1384-1542-siRNAres |
| 147025 | pGEX-6P-1-HsTNRC6Biso1_1361-1621-6His_N | Bacterial Expression of HsTNRC6Biso1_1361-1621 |
| 147026 | pGEX-6P-1-HsTNRC6Biso1_1361-1723-del1395-1534-6His_N | Bacterial Expression of HsTNRC6Biso1_1361-1723-del1395-1534 |