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GIGYF1/2 proteins use auxiliary sequences to selectively bind to 4EHP and repress target mRNA expression.

Peter D, Weber R, Sandmeir F, Wohlbold L, Helms S, Bawankar P, Valkov E, Igreja C, Izaurralde E
Genes Dev. 2017 Jun 1;31(11):1147-1161. doi: 10.1101/gad.299420.117. Epub 2017 Jul 11. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
148144pT7-V5-SBP-C1-Hs4EBP1-L75AV81A_ZMammalian Expression of Hs4EBP1-L75AV81A
148145pT7-V5-SBP-C1-Hs4EBP1-Y54AL59A_ZMammalian Expression of Hs4EBP1-Y54AL59A
148146pT7-V5-SBP-C1-Hs4EBP1-YLLVtoAAAA_ZMammalian Expression of Hs4EBP1-YLLVtoAAAA
148147pT7-V5-SBP-C1-Hs4EBP3-L61AV67A_ZMammalian Expression of Hs4EBP3-L61AV67A
148148pT7-V5-SBP-C1-Hs4EBP3-Y40AL45A_ZMammalian Expression of Hs4EBP3-Y40AL45A
148149pT7-V5-SBP-C1-Hs4EBP3-YLLVtoAAAA_ZMammalian Expression of Hs4EBP3-YLLVtoAAAA
148549pLNHA-C1-Hs4EHP_AEMammalian Expression of Hs4EHP
148550pLNHA-C1-HsGIGYF2_AEMammalian Expression of HsGIGYF2
148551pLNHA-Hs4EHP-S99N_AEMammalian Expression of Hs4EHP-S99N
148552pnEA-NpM-HsGIGYF1_33-52-GB1_AEBacterial Expression of HsGIGYF1_33-52
148553pnEA-NpM-HsGIGYF1_33-71_AEBacterial Expression of HsGIGYF1_33-71
148554pnEA-NpM-HsGIGYF1_33-71-GB1_AEBacterial Expression of HsGIGYF1_33-71
148555pnEA-NpM-HsGIGYF1_33-103-E44AE45FQ87A_AEBacterial Expression of HsGIGYF1_33-103-E44AE45FQ87A
148556pnEA-NpM-HsGIGYF1_33-103-E44AE45FQ87A-GB1_AEBacterial Expression of HsGIGYF1_33-103-E44AE45FQ87A
148557pnEA-NpM-HsGIGYF1_33-103-GB1_AEBacterial Expression of HsGIGYF1_33-103
148558pnEA-NpM-HsGIGYF2_35-54-GB1_AEBacterial Expression of HsGIGYF2_35-54
148559pnEA-NpM-HsGIGYF2_35-72-GB1_AEBacterial Expression of HsGIGYF2_35-72
148560pnEA-NpM-HsGIGYF2_35-105-GB1_AEBacterial Expression of HsGIGYF2_35-105
148561pnYC-CvH-Hs4EHP_1-234_AEBacterial Expression of Hs4EHP_1-234
148562pnYC-CvH-Hs4EHP_1-234-I85AM101A_AEBacterial Expression of Hs4EHP_1-234-I85AM101A
148563pnYC-CvH-Hs4EHP_1-234-W95A_AEBacterial Expression of Hs4EHP_1-234-W95A
148564pnYC-CvH-Hs4EHP_52-234opt_AEBacterial Expression of Hs4EHP_52-234opt
148565pnYC-CvH-Hs4EHP_52-234opt-R103LE149L_AEBacterial Expression of Hs4EHP_52-234opt-R103LE149L
148566pnYC-NpH-Hs4EHP_52-234opt_AEBacterial Expression of Hs4EHP_52-234opt
148567pnYC-NpH-Hs4EHP_52-234opt-Q159SM161DR202E_AEBacterial Expression of Hs4EHP_52-234opt-Q159SM161DR202E
148569pT7-EGFP-C1-HsGIGYF1_AEMammalian Expression of HsGIGYF1
148570pT7-EGFP-C1-HsGIGYF1_1-177_AEMammalian Expression of HsGIGYF1_1-177
148571pT7-EGFP-C1-HsGIGYF1_1-177-E86AN95F_AEMammalian Expression of HsGIGYF1_1-177-E86AN95F
148572pT7-EGFP-C1-HsGIGYF1_1-177-L60DF65DV68D_AEMammalian Expression of HsGIGYF1_1-177-L60DF65DV68D
148573pT7-EGFP-C1-HsGIGYF1_1-177-LFVPLtoDDDDA_AEMammalian Expression of HsGIGYF1_1-177-LFVPLtoDDDDA
148574pT7-EGFP-C1-HsGIGYF1_1-177-P76DL77A_AEMammalian Expression of HsGIGYF1_1-177-P76DL77A
148575pT7-EGFP-C1-HsGIGYF1_1-177-YYMLtoAAAA_AEMammalian Expression of HsGIGYF1_1-177-YYMLtoAAAA
148576pT7-EGFP-C1-HsGIGYF1-Y39AY41AM46AL47A_AEMammalian Expression of HsGIGYF1-Y39AY41AM46AL47A
148577pT7-EGFP-C1-HsGIGYF2_AEMammalian Expression of HsGIGYF2
148578pT7-EGFP-C1-HsGIGYF2_1-180_AEMammalian Expression of HsGIGYF2_1-180
148579pT7-EGFP-C1-HsGIGYF2_1-180-P78DL79A_AEMammalian Expression of HsGIGYF2_1-180-P78DL79A
148580pT7-EGFP-C1-HsGIGYF2_1-180-YYMLtoAAAA_AEMammalian Expression of HsGIGYF2_1-180-YYMLtoAAAA
148581pT7-EGFP-HsGIGYF1_1-177-LFVENtoDDDAF_AEMammalian Expression of HsGIGYF1_1-177-LFVENtoDDDAF
148582pT7-EGFP-HsGIGYF2_1-180-LFItoDDD_AEMammalian Expression of HsGIGYF2_1-180-LFItoDDD
148583pT7-V5-SBP-C1-Hs4EHP-I85AM101A_AEMammalian Expression of Hs4EHP-I85AM101A
148584pT7-V5-SBP-C1-Hs4EHP-I85AW95AM101A_AEMammalian Expression of Hs4EHP-I85AW95AM101A
148585pT7-V5-SBP-C1-Hs4EHP-R103LE149L_AEMammalian Expression of Hs4EHP-R103LE149L
148587pT7-V5-SBP-C1-Hs4EHP-W95AR103LE149L_AEMammalian Expression of Hs4EHP-W95AR103LE149L
148588pT7-V5-SBP-Hs4EHP_AEMammalian Expression of Hs4EHP
148686pnEA-NpM-HsGIGYF2_35-105-E46AE47FN91A-GB1_AFBacterial Expression of HsGIGYF2_35-105-E46AE47FN91A
148687pT7-EGFP-C1-Hs4E-T_AFMammalian Expression of Hs4E-T
148688pT7-EGFP-C1-HsGIGYF2_1-180-E88AN97F_AFMammalian Expression of HsGIGYF2_1-180-E88AN97F
148689pT7-EGFP-C1-HsGIGYF2_1-180-LFIENtoDDDAF_AFMammalian Expression of HsGIGYF2_1-180-LFIENtoDDDAF
148690pT7-EGFP-C1-HsGIGYF2_1-180-LFIPLtoDDDDA_AFMammalian Expression of HsGIGYF2_1-180-LFIPLtoDDDDA
148691pT7-EGFP-C1-HsGIGYF2-YYMLtoAAAA_AFMammalian Expression of HsGIGYF2-YYMLtoAAAA
148692pT7-V5-SBP-C1-Hs4EHP-S99N_AFMammalian Expression of Hs4EHP-S99N
148878pCIneo-luc-TNFalpha-ARE-polyA-Malat_AHMammalian Expression of TNFalpha-ARE-polyA-Malat
148879pLNHA-C1-HsTTP_1-313_AHMammalian Expression of HsTTP_1-313

Antibodies from Article