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Distribution and phasing of sequence motifs that facilitate CRISPR adaptation.
Santiago-Frangos A, Buyukyoruk M, Wiegand T, Krishna P, Wiedenheft B
Curr Biol. 2021 Aug 23;31(16):3515-3524.e6. doi: 10.1016/j.cub.2021.05.068. Epub 2021 Jun 25. PubMed Article

Plasmids from Article

ID Plasmid Purpose
149384pHisIHFαIHFβExpression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbeta
149385pStrepIHFbExpression of P. aeruginosa PA14, StrepII-HRV3C-IHFbeta
149386pCRISPR2_wtP. aeruginosa PA14 CRISPR2 locus
149387pCRISPR2_-6DP. aeruginosa PA14 CRISPR2 locus, with 6bp deletion downstream of IHFprox site
149388pCRISPR2_-5DP. aeruginosa PA14 CRISPR2 locus, with 5bp deletion downstream of IHFprox site
149389pCRISPR2_+1DP. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox site
149390pCRISPR2_+5DP. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox site
149391pCRISPR2_+10DP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream of IHFprox site
149393pCRISPR2_+10D+7UP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox site
149394pCRISPR2_+10D+10UP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox site
149395pCRISPR2_IHF_OptP. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site
149396pCRISPR2_IHF_MutP. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF
149397pCRISPR2_motif_scramP. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motif
162318pCRISPR2_IHFdist_rmP. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removed
162319pCRISPR2_IRdist_scramP. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motif

Antibodies from Article